CD223 (Lag-3) Rat anti-Mouse, PE, Clone: eBioC9B7W (C9B7W), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-2231-83

200UG ANTI-MOUSE CD223 (LAG-3) PE

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15268729

Quantity Price
1 £ 188.0 / 200µg
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

Antigen CD223 (Lag-3)
Applications Flow Cytometry
Clone eBioC9B7W (C9B7W)
Concentration 0.2mg/mL
Conjugate PE
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Isotype IgG1
Quantity 200μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54kDa fragment containing all the extracellular domains, and a 16kDa fragment containing the intracellular and transmembrane domains. The 54kDa can remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.