CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15588056	Affymetrix eBioscience 17-2231-80	25 μg
15598056	Affymetrix eBioscience 17-2231-82	100 μg

Description

Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragment containing all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223. CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function. Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

CD223 (LAG-3) Monoclonal antibody specifically detects CD223 (LAG-3) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD223 (LAG-3)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3lymphocyte activating 3; soluble LAG3
• Gene Symbols: LAG3
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: LAG3

• Clone: eBioC9B7W (C9B7W)
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q61790
• Gene ID (Entrez): 16768
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.