CD223 (LAG-3) Monoclonal Antibody (3DS223H), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15319965	Affymetrix eBioscience 48-2239-42	100 Tests
15529736	Affymetrix eBioscience 48-2239-41	25 Tests

Description

Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells). This 3DS223H antibody will recognize a formaldehyde-fixed epitope. Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm).

CD223 (LAG-3) Monoclonal antibody specifically detects CD223 (LAG-3) in Human samples. It is validated for Flow Cytometry.

Specifications

• Clone: 3DS223H
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3902
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P18627
• Gene Symbols: LAG3
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: LAG3

For Research Use Only.