CD217 (IL-17Ra), APC, clone: PAJ-17R, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 17-7182-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibody PAJ-17R recognizes mouse IL-17 receptor A (IL-17RA) also known as CD217. This single transmembrane domain-containing receptor can homo- or heterodimerize with IL-17RC or IL-17RB. IL-17RA binds to IL-17A, IL-17F(cytokines of the Th 17 lineage), or IL-17E (IL-25). Ligand binding to the single subunit of IL-17RA has been shown to induce homo- or heterodimerization of the receptor complex that in turn recruits Act-1 via the SEFIR domains leading to recruitment of TRAF6 and signaling through the NFkappaB and MAPK pathways.Initial reports suggested ubiquitous expression of IL-17RA based on binding studies with IL-17-Fc and expression of mRNA. Interestingly, despite this, not all cells expressing the receptor respond to IL-17A. In particular, resting T cells do not respond. It is not known if the expression level of IL-17RA protein varies or if some cells express the receptor but are not able to activate downstream signaling events. Recent evidence suggests a more limited expression profile based on IL-17RA knock-out mice which display decreased numbers of neutrophils with impaired function in response to challenge with K. pneumonia or Candida albicans. This suggests IL-17RA plays a more dominant and critical role in neutrophil development and function. Our studies suggest that expression can be found on abundantly on Gr-1^dim population and many of the Gr-1^bright bone marrow cells, as well as some F4/80 positive cells. This staining with PAJ-17R is not detected in IL-17RA knock-out mice.Applications Reported: This PAJ-17R antibody has been reported for use in flow cytometric analysis.Applications Tested: This PAJ-17R antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD217 (IL-17 Receptor A)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|IL-17 alpha receptor, IL-17R, IL-17RA|
For Research Use Only.