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CD215 (IL-15Ra), PE, clone: DNT15Ra, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7149-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15269299

  • £87.25 / 25µg

Estimated Shipment date
from Supplier 26-04-2018
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Description: This DNT15Ra antibody reacts with the alpha subunit of the Mouse IL-15 Receptor. The IL-15 Receptor alpha subunit is unique to IL-15, while the other two subunits of the receptor, beta and gamma, are shared with IL-2. IL-15R signaling is mediated by Jak/STAT pathways, and results in the activation of neutrophils, dendritic cells, and macrophages. IL-15 has also been found to play a critical role in the development and survival of NK cells and CD8+ T cells.Although high levels of mouse IL-15 mRNA have been observed in many cell types, free IL-15 is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and only secreted in complex with IL-15 Receptor alpha. This chaperoning is necessary from the ER to the Golgi, through secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta-gamma subunits of the receptor, or can be secreted in a soluble form lacking the transmembrane domain of IL-15 Receptor alpha. IL-15 and IL-15 Receptor alpha are expressed by dendritic cells and macrophages in response to inflammatory stimuli.Our testing suggests that there may be strain differences in expression and staining of the Mouse IL-15 Receptor alpha. This DNT15Ra antibody has been tested on cells from C57Bl/6 mice. Staining has also been observed with this antibody after pre-incubation of NK cells with exogenous Mouse IL-15/IL-15R Complex, suggesting that DNT15Ra will recognize both Mouse IL-15 Receptor alpha and the Mouse IL-15/IL-15R Complex.Applications Reported: This DNT15Ra antibody has been reported for use in flow cytometric analysis.Applications Tested: This DNT15Ra antibody has been tested by flow cytometric analysis of mouse dendriric cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.


CD215 (IL-15 Receptor alpha)
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IL-15RA, Interleukin-15 receptor subunit alpha, IL15RA, CD215
IgG1, kappa
Flow Cytometry
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.