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CD215 (IL-15Ra), clone: eBioJM7A4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7159-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278027

  • £43.20 / 25µg


Estimated Shipment
26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL-2Rbeta and gamma chains, which are essential for signal transduction. The IL-15Ralpha receptor is structurally related to IL-2Ralpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2Ralpha and IL-2. In the human, 3 differentially spliced human IL-15Ralpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL-15Ralpha, like that of IL-2Ralpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL-2Rbeta and IL-2Rgamma in the absence of the alpha subunit.Applications Reported: This eBioJM7A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).Applications Tested: This eBioJM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN gamma-stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD215 (IL-15 Receptor alpha)
eBioJM7A4
Unconjugated
Purified
Murine
25μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-15 Receptor alpha, IL-15R, IL15R
IgG2b
RUO
Primary
Human
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