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CD21, PerCP-eFluor™ 710, clone: HB5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0219-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15559096

  • £199.83 / 100 tests


EU Stock 1
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Description

Description

Description: The HB5 monoclonal antibody reacts with human CD21, an approximately 145kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.Applications Reported: This HB5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HB5 antibody has been pre-titrated and tested by flow cyometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD21
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P20023
EBV receptor, complement C3d receptor, complement receptor type 2, epstein-Barr virus receptor
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HB5
PerCP-eFluor 710
CR2
Conjugated
CR2
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
1380
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