CD21/CD35 Rat anti-Mouse, eFluor 450, Clone: eBio4E3 (4E3), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0212-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15311700

Quantity Price
1 £ 78.88 / 25µg
Estimated Shipment date
from Supplier 03-04-2017
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Description and Specification

Specification

Host Species Rat
Format Conjugated
Clone eBio4E3 (4E3)
Isotype IgG2a
Monoclonal or Polyclonal Monoclonal
Primary or Secondary Primary
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Species Reactivity Mouse
Gene Alias CR2/CR1, C3DR
Conjugate eFluor 450
Regulatory Status RUO
Quantity 25μg
Applications Flow Cytometry
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Antigen CD21/CD35
Concentration 0.2mg/mL

The monoclonal antibody eBio4E3 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which result in cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is little evidence which demonstrates their expression on macrophages. CD21 is a receptor for complement component C3d and Epstein-Barr virus (EBV), and in association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2 deficient mice display impaired inflammatory and humoral immune responses in vivo. The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other suggesting that they bind to different epitopes.