CD21/CD35 Monoclonal Antibody (eBio4E3 (4E3)), eFluor™ 450, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0212-82

Product Code. 15321700

Description

Description: The monoclonal antibody eBio4E3 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which result in cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is little evidence which demonstrates their expression on macrophages. CD21 is a receptor for complement component C3d and Epstein-Barr virus (EBV), and in association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2 deficient mice display impaired inflammatory and humoral immune responses in vivo. The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other suggesting that they bind to different epitopes. Applications Reported: This eBio4E3 (4E3) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4E3 (4E3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

CD21/CD35 Monoclonal antibody specifically detects CD21/CD35 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD21/CD35
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P19070
• Gene Symbols: Cr2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12902
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBio4E3 (4E3)
• Conjugate: eFluor 450
• Gene: Cr2
• Gene Alias: C3b/C4b receptor; C3-binding protein; C3DR; CD21; CD35; CD35 antigen; Complement C3d receptor; complement component (3d/Epstein Barr virus) receptor 2; complement component 3d receptor 2; complement receptor 2; complement receptor type 1; complement receptor type 2; CR; Cr1; Cr-1; Cr2; Cr-2; CVID7; EBV receptor; Epstein-Barr virus receptor; expressed in B lymphocytes; SLEB9
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a λ

For Research Use Only.