CD21/CD35 Rat anti-Mouse, APC-eFluor 780, Clone: eBio8D9, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 47-0211-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15341430

Quantity Price
1 £ 72.77 / 25µg
Estimated Shipment date
from Supplier 03-04-2017
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Quantity 25μg
Regulatory Status RUO
Species Reactivity Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias CR2/CR1, C3DR
Conjugate APC-eFluor 780
Host Species Rat
Clone eBio8D9
Applications Flow Cytometry
Antigen CD21/CD35
Isotype IgG2a
Format Conjugated
Monoclonal or Polyclonal Monoclonal
Concentration 0.2mg/mL
Primary or Secondary Primary

The monoclonal antibody eBio8D9 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which produce cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is some evidence which demonstrates their expression on macrophages. CD21 is a receptor for the complement component C3d and Epstein-Barr virus (EBV). In association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2-deficient mice display impaired inflammatory and humoral immune responses in vivo.

The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other, suggesting that they bind to different epitopes.