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CD209 (DC-SIGN), FITC, clone: LWC06, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-2092-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15218199

  • £168.34 / 100µg


Estimated Shipment date
from Supplier 30-07-2018
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Description

Description

Description: The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8 alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a calcium dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.CD209 protein is sensitive to collagenase treatment.Applications Reported: This LWC06 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LWC06 antibody has been tested by flow cytometric analysis of mouse CIRE/DC-SIGN-transfected CHO cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD209 (DC-SIGN)
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q91ZX1
CD209, CDSIGN, CIRE, DC-SIGN, DC-SIGN1, Dcsign, MGC130443, SIGN-R1, SIGNR5, CD209a
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
LWC06
FITC
Cd209a
Conjugated
Cd209a
IgG2a
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
170786
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