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CD209 (DC-SIGN), PE-Cyanine7, clone: eB-h209, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-2099-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15588516

  • £186.79 / 100 tests


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.Applications Reported: This eB-h209 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte derived dendritic cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD209 (DC-SIGN)
eB-h209
PE-Cyanine7
Conjugated
Rat
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Clec4L
IgG2a
RUO
Primary
Human
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