CD209a, eFluor 660, clone: MMD3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-2094-80

Product Code. 15351890

Description

Description: This MMD3 monoclonal antibody recognizes mouse CD209a, which is also known as DC-SIGN. CD209a is a type II transmembrane C-type lectin expressed on a subset of dendritic cells, including some CD4+, CD8- and plasmacytoid pre-dendritic cells. Studies indicate that CD209a expression can vary according to the activation state of the host. Moreover, CD209a is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. CD209a is involved in mediating the innate immune response by binding microbial carbohydrates.Cross-blocking studies suggest that MMD3 recognizes a different a different epitope from LWC06 and 5H10.Applications Reported: This MMD3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MMD3 antibody has been tested by flow cytometric analyis of transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD209 (DC-SIGN), CD209a
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q91ZX1
• Gene Alias: CD209, CDSIGN, CIRE, DC-SIGN, DC-SIGN1, Dcsign, MGC130443, SIGN-R1, SIGNR5, CD209a
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow CytometryþImmunohistochemistry (Frozen)
• Clone: MMD3
• Conjugate: eFluor 660
• Gene: Cd209a
• Format: Conjugated
• Gene Symbols: Cd209a
• Isotype: IgG2c, kappa
• Quantity: 25μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 170786

For Research Use Only.