CD209a, eFluor 660, clone: MMD3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-2094-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This MMD3 monoclonal antibody recognizes mouse CD209a, which is also known as DC-SIGN. CD209a is a type II transmembrane C-type lectin expressed on a subset of dendritic cells, including some CD4+, CD8- and plasmacytoid pre-dendritic cells. Studies indicate that CD209a expression can vary according to the activation state of the host. Moreover, CD209a is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. CD209a is involved in mediating the innate immune response by binding microbial carbohydrates.Cross-blocking studies suggest that MMD3 recognizes a different a different epitope from LWC06 and 5H10.Applications Reported: This MMD3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MMD3 antibody has been tested by flow cytometric analyis of transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|CD209, CDSIGN, CIRE, DC-SIGN, DC-SIGN1, Dcsign, MGC130443, SIGN-R1, SIGNR5, CD209a|
|Flow CytometryþImmunohistochemistry (Frozen)|
|IgG2c, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.