CD207 (Langerin) Rat anti-Mouse, Clone: eBioL31, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-2075-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15257307

Quantity Price
1 £ 152.25 / 100µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Host Species Rat
Conjugate Unlabeled
Antigen CD207 (Langerin)
Species Reactivity Mouse
Isotype IgG2a
Applications Flow Cytometry
Applications Flow Cytometry (Intracellular Staining)
Applications Immunohistochemistry (Frozen)
Applications Immunoprecipitation
Applications Western Blotting
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Quantity 100μg
Format Purified
Gene Alias Clec4K
Clone eBioL31
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Storage Requirements Store at 2-8°C.
Concentration 0.5mg/mL
Regulatory Status RUO

The eBioL31 monoclonal antibody reacts with mouse CD207, also known as Langerin, which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). Mouse CD207 is a 48kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake. LC are located in the epidermis and upon activation, reduce CD207 expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines.

Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse CD207 cDNA. Preliminary data from mouse CD207 cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3/Transcription Factor Staining Bufer Set (cat. 00-5523). The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin. Differences in expression has been observed between mouse strains.