CD207 (Langerin) Rat anti-Mouse, Alexa Fluor 488, Clone: eBioRMUL.2, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 53-2073-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15382010

Quantity Price
1 £ 72.77 / 25µg
EU Stock 2
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Description and Specification

Specification

Format Conjugated
Clone eBioRMUL.2
Primary or Secondary Primary
Conjugate Alexa Fluor 488
Quantity 25μg
Antigen CD207 (Langerin)
Applications Flow Cytometry (Intracellular Staining)
Gene Alias Clec4K
Isotype IgG2a
Monoclonal or Polyclonal Monoclonal
Regulatory Status RUO
Concentration 0.5mg/mL
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.

eBioRMUL.2 is a monoclonal antibody that reacts with the predicted extracellular domain of mouse CD207, also known as Langerin. Mouse CD207 is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). Mouse CD207 is a 48 kDa, C-type lectin and putative transmembrane protein that likely plays a role in antigen recognition and uptake. LC are located in the epidermis and upon activation reduce expression of CD207 and begin migrating through the dermis towards lymphatic vessels. Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines.

Western Blotting with RMUL.2 reveals a 48kDa band in lysate from mouse ear epidermis as well as in lysates from cells transfected with mouse CD207 cDNA. Cross reactivity of this antibody with other proteins has not been determined. Preliminary data from mouse CD207 cDNA transfected cells suggests that RMUL.2 can be used for intracellular staining followed by flow cytometric analysis. We have observed in our transfectants the majority of protein is expressed intracellularly with a small percentage on the cell surface. In addition, RMUL.2 has also been tested by immunohistology on mouse ear epidermal sheets and shows specific staining.