CD205 Monoclonal Antibody (205yekta), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-2051-42

Product Code. 15548516

Description

Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells. Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis. Applications Tested: This 205yekta antibody has been pre-titrated and tested by flow cytometric analysis of in vitro-cultured bone marrow-derived dendritic cells or mouse Granulocytes. This can be used at 5 μL (0.007 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat.

CD205 Monoclonal antibody specifically detects CD205 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD205
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q60767
• Gene Symbols: LY75
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17076
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 205yekta
• Conjugate: PE-Cyanine7
• Gene: LY75
• Gene Alias: CA-IX; CD205; CLEC13B; C-type lectin domain family 13 member B; DCL-1 fusion; DEC205; DEC-205; DEC-205/CD205 protein; gp200; gp200-MR6; group VI C-type animal lectin; LY75; ly-75; Lymphocyte antigen 75; membrane antigen MN; P54/58N; pMW1; RGD1565962; RP23-10L17.4
• Host Species: Rat
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.