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CD205, PE-Cyanine7, clone: 205yekta, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-2051-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15548516

  • £186.95 / 100 tests


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis.Applications Tested: This 205yekta antibody has been pre-titrated and tested by flow cytometric analysis of in vitro-cultured bone marrow-derived dendritic cells or mouse Granulocytes. This can be used at 5 μL (0.007 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD205
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q60767
RP23-10L17.4, CD205, DEC-205, ly-75, CLEC13B, GP200-MR6, DCL-1 fusion, LY75-CD302 fusion protein
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
205yekta
PE-Cyanine7
Ly75
Conjugated
Ly75
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
17076
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