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CD20 Monoclonal Antibody (2H7), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£99.00 - £231.00

Specifications

Antigen CD20
Clone 2H7
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Product Code Brand Quantity Price Quantity & Availability  
15548366
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Affymetrix eBioscience
25-0209-42
100 Tests
£231.00
100 tests
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15538366
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Affymetrix eBioscience
25-0209-41
25 Tests This item is currently unavailable or has been discontinued.
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Description

Description

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation. Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

CD20 Monoclonal antibody specifically detects CD20 in Human samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD20
5 μL/Test
Monoclonal
Liquid
RUO
P11836
IgG2b κ
Affinity chromatography
Antibody
2H7
Flow Cytometry
PE-Cyanine7
Mouse
Human
931
Primary
4° C, store in dark, DO NOT FREEZE!
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