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CD20, PE-Cyanine5.5, clone: 2H7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 35-0209-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15528856

  • £76.10 / 25 tests


Estimated Shipment date
from Supplier 26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-567 nm; Emission: 690 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Specifications

Specifications

CD20
2H7
PE-Cyanine5.5
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B1, Leu-16
IgG2b
RUO
Primary
Human
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