CD20 Monoclonal Antibody (L26), eFluor™ 615, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15371090	Affymetrix eBioscience 42-0202-80	25 μg
15381090	Affymetrix eBioscience 42-0202-82	100 μg

Description

Description: The monoclonal antibody L26 recognizes human CD20, a two transmembrane spanning protein found on precursor and mature B lymphocytes. Expression is lost upon differentiation to plasma cells. The levels of CD20 are critical in evaluating maligt B lymphocytes including Non-Hodgkin's Lymphoma, and Acute B cell, Chronic Lymphocytic and Hairy-Cell Leukemias. CD20 plays a role in B cell differentiation and activation. The structure of the protein shows a small extracellular loop with the carboxy and amino termini in the cytoplasmic compartment. The L26 antibody recognizes an epitope in the cytoplasmic domain and therefore requires permeabilization of the tissue for staining applications. Unlike many anti-CD20 antibodies that recognize the extracellular domain, the epitope recognized by L26 will not be blocked by therapeutic antibodies to CD20, like Rituximab. Applications Reported: This L26 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P). Applications Tested: This L26 antibody has been tested immunohistochemistry on FFPE human tonsil with low pH antigen retrieval. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

CD20 Monoclonal antibody specifically detects CD20 in Cynomolgus Monkey, Human, Mouse, Rhesus Monkey samples. It is validated for Immunocytochemistry, Immunohistochemistry (Paraffin).

Specifications

• Antigen: CD20
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 615
• Host Species: Mouse
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: L26
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.