CD2, Functional Grade, clone: RM2-5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0021-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The RM2-5 monoclonal antibody reacts with the mouse CD2 molecule, an approximately 50-55kDa cell surface receptor expressed by all mouse lymphocytes. Expression of the CD2 antigen in the mouse differs from that of the human in that B lineage cells in the mouse from the pre-B cell stage to mature B cells express CD2. CD2 is a ligand for CD48 in the mouse and is involved in adhesion and activation of T cells.Applications Reported: The RM2-5 antibody has been reported for use in flow cytometric analysis. RM2-5 has also been reported in in vitro blocking of mouse CD2 (LFA-2).Applications Tested: The RM2-5 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|aqueous buffer, no sodium azide|
|Store at 2-8°C.|