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CD2, PerCP-eFluor™ 710, clone: RPA-2.10, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0029-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15549056

  • £64.81 / 25 tests


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.RPA-2.10 crossreacts to non-human primates and pigs.Applications Reported: This RPA-2.10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD2
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P06729
CD2 antigen, sheep red blood cell receptor, LFA-3 receptor, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, erythrocyte receptor, lymphocyte-function antigen-2, rosette receptor
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
BaboonþChimpanzeeþCynomolgusþHumanþPorcineþRhesus
Flow Cytometry
RPA-2.10
PerCP-eFluor 710
CD2
Conjugated
CD2
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
914
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