CD2, Functional Grade, clone: RPA-2.10, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0029-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.RPA-2.10 crossreacts to non-human primates and pigs.Applications Reported: The RPA-2.10 antibody been reported for use in flow cytometric analysis. RPA-2.10 has also been reported in blocking of CD2 activity in in vitro functional assays.Applications Tested: This RPA-2.10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|CD2 antigen, sheep red blood cell receptor, LFA-3 receptor, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, erythrocyte receptor, lymphocyte-function antigen-2, rosette receptor|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.