missing translation for 'onlineSavingsMsg'
Learn More

CD2 Monoclonal Antibody (RPA-2.10), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0029-81

65.16 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : Z2

Additional Details : Weight : 0.01000kg

Product Code. 15218337

  • £97.75 / 50µg
Estimated Shipment: 05-04-2024
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction. RPA-2.10 crossreacts to non-human primates and pigs. Applications Reported: The RPA-2.10 antibody been reported for use in flow cytometric analysis. RPA-2.10 has also been reported in blocking of CD2 activity in in vitro functional assays. Applications Tested: This RPA-2.10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.
Specifications

Specifications

CD2
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
P06729, Q6SZ61
CD2
Affinity chromatography
RUO
100126712, 102120798, 396662, 449512, 677855, 914
4° C
Liquid
Flow Cytometry, Functional Assay, Neutralization
RPA-2.10
Functional Grade
CD2
Cd2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; CD2R; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA2; LFA-2; LFA-3 receptor; Ly37; Ly-37; lymphocyte antigen 37; OX34; OX-34; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; Rosette receptor; SRBC; surface immunoglobulin superfamily molecule; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
Mouse
50 μg
Primary
Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human Primate, Porcine, Rhesus Monkey
Antibody
IgG1 κ
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only.