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CD199 (CCR9), eFluor 450, clone: eBioCW-1.2 (CW-1.2), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-1991-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15321760

  • £80.80 / 25µg


Estimated Shipment date
from Supplier 05-06-2018
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Description

Description

Description: The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells.Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis or mouse thymocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD199
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9WUT7
CDw199, GPR-9-6, GPR28
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBioCW-1.2 (CW-1.2)
eFluor 450
Ccr9
Conjugated
Ccr9
IgG2a
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12769
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