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CD197 (CCR7), PE, clone: 3D12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-1979-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15576936

  • £62.12 / 25 tests


EU Stock 7
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Description

Description

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD197 (CCR7)
3D12
PE
Conjugated
Rat
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
EBI-1, MIP-3 beta Receptor
IgG2a, kappa
RUO
Primary
Human
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