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CD197 (CCR7), Biotin, clone: 3D12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-1979-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15219769

  • £56.31 / 25µg


Estimated Shipment date
from Supplier 28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD197 (CCR7)
3D12
Biotin
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
EBI-1, MIP-3 beta Receptor
IgG2a
RUO
Primary
Human
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