CD195 (CCR5) Monoclonal Antibody (NP-6G4), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-1956-42

Product Code. 15578506

Description

Description: The NP-6G4 monoclonal antibody reacts with human CD195 (CCR5), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1a, MIP-1b and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Applications Reported: This NP-6G4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This NP-6G4 antibody has been pre-titrated and tested on normal human peripheral blood cells. It is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

CD195 (CCR5) Monoclonal antibody specifically detects CD195 (CCR5) in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD195 (CCR5)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P51681
• Gene Symbols: CCR5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1234
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: NP-6G4
• Conjugate: PE-Cyanine7
• Gene: CCR5
• Gene Alias: AM4-7; C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 (gene/pseudogene); C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5; Ccr5; CCR-5; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine (C-C) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; Cmkbr5; HIV-1 fusion coreceptor; IDDM22; MIP-1 alpha receptor
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.