CD195 (CCR5), PE, clone: eBioT21/8 (T21/8), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-1957-41
Code : NEW
Additional Details : Weight : 0.20000kg
DescriptionDescription: The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.Applications Reported: This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioT21/8 (T21/8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. For optimal performance, it is recommended that staining with eBioT21/8 be done at room temperature or 37°C for 1 hour in Flow Cytometry Staining Buffer (cat. 00-4222). This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|