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CD19, eFluor 660, clone: eBio1D3 (1D3), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0193-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15371850

  • £62.06 / 25µg


Estimated Shipment date
from Supplier 28-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD19
eBio1D3 (1D3)
eFluor 660
Conjugated
IgG2a
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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