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CD19, PerCP-eFluor™ 710, clone: J3-129, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0197-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15529096

  • £206.18 / 100 tests


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: This J3-129 monoclonal antibody reacts with human CD19, a 95-kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development, excluding terminally differentiated plasma cells. However, follicular dendritic cells express CD19. CD21, CD81, Leu13, MHC class II, and CD19 together form a multi-molecular complex that associates with the B cell receptor. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and B cell proliferation.Applications Reported: This J3-129 antibody has been reported for use in flow cytometric analysis.Applications Tested: This J3-129 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD19
J3-129
PerCP-eFluor 710
Conjugated
IgG1
RUO
Primary
Human
Flow Cytometry
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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