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CD19, eFluor 450, clone: HIB19, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0199-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15529646

  • £67.59 / 25 tests


EU Stock 2
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Description

Description

Description: The HIB19 monoclonal antibody reacts with human CD19, a 95kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: This HIB19 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of human mononuclear cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD19
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P15391
AW495831, B-lymphocyte antigen CD19, differentiation antigen CD19
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HIB19
eFluor 450
CD19
Conjugated
CD19
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
930
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