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CD185 (CXCR5), PerCP-eFluor™ 710, clone: SPRCL5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-7185-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15549356

  • £90.39 / 25µg

Estimated Shipment date
from Supplier 01-05-2018
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Description: This SPRCL5 monoclonal antibody reacts with Mouse CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. CXCR5+ circulating T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with CD45RO and such activation markers as CD69 and ICOS. Tonsillar CXCR5+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CXCR5 an established marker of follicular helper T cells.This SPRCL5 does not see a formaldehyde fixed epitope, therefore staining should be done prior to fixation.Applications Reported: This SPRCL5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SPRCL5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


CD185 (CXCR5)
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
CXC Chemokine Receptor 5, Fusin, LESTR, HUMSTR
Affinity chromatography
Flow Cytometry
PerCP-eFluor 710
IgG2a, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.