CD185 (CXCR5) Rat anti-Mouse, PerCP-eFluor 710, Clone: SPRCL5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-7185-80

25UG Anti-Mouse CD185 (CXCR5) PerCP-eFluor 710

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15549356

Quantity Price
1 £ 134.0 / 25µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Antigen CD185 (CXCR5)
Applications Flow Cytometry
Clone SPRCL5
Concentration 0.2mg/mL
Conjugate PerCP-eFluor 710
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias C-X-C Chemokine Receptor Type 5, BLR1, MDR15
Host Species Rat
Isotype IgG2a
Quantity 25μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This SPRCL5 monoclonal antibody reacts with Mouse CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. CXCR5+ circulating T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with CD45RO and such activation markers as CD69 and ICOS. Tonsillar CXCR5+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CXCR5 an established marker of follicular helper T cells.

This SPRCL5 does not see a formaldehyde fixed epitope, therefore staining should be done prior to fixation.