CD185 (CXCR5) Rat anti-Mouse, PerCP-eFluor 710, Clone: SPRCL5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-7185-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15549356

Quantity Price
1 £ 87.96 / 25µg
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Clone SPRCL5
Concentration 0.2mg/mL
Gene Alias C-X-C Chemokine Receptor Type 5, BLR1, MDR15
Quantity 25μg
Applications Flow Cytometry
Species Reactivity Mouse
Conjugate PerCP-eFluor 710
Monoclonal or Polyclonal Monoclonal
Isotype IgG2a
Primary or Secondary Primary
Regulatory Status RUO
Antigen CD185 (CXCR5)
Format Conjugated
Host Species Rat

This SPRCL5 monoclonal antibody reacts with Mouse CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. CXCR5+ circulating T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with CD45RO and such activation markers as CD69 and ICOS. Tonsillar CXCR5+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CXCR5 an established marker of follicular helper T cells.

This SPRCL5 does not see a formaldehyde fixed epitope, therefore staining should be done prior to fixation.