CD185 (CXCR5) Mouse anti-Non-human primate, PE, Clone: 87.1, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-1859-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15566926

Quantity Price
1 £ 78.88 / 25 tests
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Conjugate PE
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Species Reactivity Non-human primate
Concentration 5μL (0.125μg)/test
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Quantity 25 tests
Isotype IgG1
Monoclonal or Polyclonal Monoclonal
Clone 87.1
Applications Flow Cytometry
Regulatory Status RUO
Gene Alias C-X-C chemokine receptor type 5, BLR1, MDR15
Host Species Mouse
Primary or Secondary Primary
Antigen CD185 (CXCR5)

This 87.1 monoclonal antibody reacts with non-human primate (specifically rhesus) CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), and a subpopulation of memory (CD45RO+) T cells. CD185+ circulating T cells are in a resting state and migrate to the lymph nodes due to CCR7 and CD62L expression. In tonsils, CD185 is expressed on nearly all CD4+ cells along with CD45RO and the activation markers CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular CXCL13-induced T cell migration into the B cell follicles of germinal centers. Thus, CD185 is an established marker of follicular helper T cells (Tfh). In rhesus macaques, infection with simian immunodeficiency virus (SIV) has been reported to increase the number of germinal centers and Tfh cells within the lymph nodes.

Refer to the rhesus reactive alternative clone MU5UBEE (cat. 12-9185) for identification of CXCR5 on T cells in addition to B cells.