CD185 (CXCR5), PE, clone: 87.1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-1859-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This 87.1 monoclonal antibody reacts with non-human primate (specifically rhesus) CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), and a subpopulation of memory (CD45RO+) T cells. CD185+ circulating T cells are in a resting state and migrate to the lymph nodes due to CCR7 and CD62L expression. In tonsils, CD185 is expressed on nearly all CD4+ cells along with CD45RO and the activation markers CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular CXCL13-induced T cell migration into the B cell follicles of germinal centers. Thus, CD185 is an established marker of follicular helper T cells (Tfh). In rhesus macaques, infection with simian immunodeficiency virus (SIV) has been reported to increase the number of germinal centers and Tfh cells within the lymph nodes.Refer to the rhesus reactive alternative clone MU5UBEE (cat. 12-9185) for identification of CXCR5 on T cells in addition to B cells.Applications Reported: This 87.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 87.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal rhesus peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|CXC Chemokine Receptor 5, Fusin, LESTR, HUMSTR|
For Research Use Only.