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CD185 (CXCR5), PerCP-eFluor™ 710, clone: MU5UBEE, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9185-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15549406

  • £214.34 / 100 tests


EU Stock 3
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Description

Description

Description: The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. Circulating CD185+ T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CD185 is expressed on nearly all CD4+ cells together with CD45RO and activation markers such as CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing help to B cells. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CD185 an established marker of follicular helper T cells.The MU5UBEE monoclonal antibody also crossreacts with Rhesus macaque.Applications Reported: This MU5UBEE antibody has been reported for use in flow cytometric analysis.Applications Tested: This MU5UBEE antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD185 (CXCR5)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P32302
CXC Chemokine Receptor 5, Fusin, LESTR, HUMSTR
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþNon-human PrimateþRhesus
Flow Cytometry
MU5UBEE
PerCP-eFluor 710
CXCR5
Conjugated
CXCR5
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
643
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