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CD184 (CXCR4), Functional Grade, clone: 12G5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9999-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278857

  • £93.52 / 50Āµg


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Description

Description

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.Applications Reported: The 12G5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CXCR4 in functional studies. (Fluorochrome-conjugated 12G5 is recommended for use in flow cytometry.).Applications Tested: The 12G5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD184 (CXCR4)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P61073
CXC Chemokine Receptor 4, Fusin, LESTR, HUMSTR
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryĆ¾Functional Assay
12G5
Functional Grade
CXCR4
Purified
CXCR4
IgG2a, kappa
50μg
Store at 2-8°C.
Primary
7852
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