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CD182 (CXCR2), PerCP-eFluor™ 710, clone: ebio5E8-C7-F10 (5E8-C7-F10), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-1829-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15569206

  • £77.01 / 25 tests


Estimated Shipment date
from Supplier 29-05-2018
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Description

Description

Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8R beta). CD182 is a 67-70kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8R alpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.Applications Reported: This ebio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This ebio5E8-C7-F10 (5E8-C7-F10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD182 (CXCR2)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P25025
CXCR2, CDw128b, CD182 antigen, CMKAR2, CXCR-2, CD182, CXC-R2, IL-8R B, Il-8 receptor b, IL-8 receptor type 2, Il-8rb, IL8R2, IL8RB, IL-8 receptor beta
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
ebio5E8-C7-F10 (5E8-C7-F10)
PerCP-eFluor 710
CXCR2
Conjugated
CXCR2
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3579
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