CD181 (CXCR1), FITC, clone: eBio8F1-1-4 (8F1-1-4), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-1819-41

Product Code. 15566506

Description

CXCR1 is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36.

Description: The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.Applications Reported: This eBio8F1-1-4 (8F1-1-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio8F1-1-4 (8F1-1-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD181 (CXCR1)
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P25024
• Gene Symbols: CXCR1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3577
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBio8F1-1-4 (8F1-1-4)
• Conjugate: FITC
• Gene: CXCR1
• Gene Alias: C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA, IL-8 Receptor alpha, IL-RA
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.