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CD178 (Fas Ligand), Functional Grade, clone: MFL3, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5911-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15218697

  • £106.54 / 100Āµg


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Description

Description

Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.Applications Reported: MFL3 has been reported for use in flow cytometric analysis. It has also been reported in blocking of the FasL mediated killing in functional assays.Applications Tested: The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD178 (Fas Ligand)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P41047
FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1, Fas Ligand, Fas-LG
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryĆ¾Functional Assay
MFL3
Functional Grade
Fasl
Purified
Fasl
IgG
100μg
Store at 2-8°C.
Primary
14103
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