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CD178 (Fas Ligand), PE, clone: NOK-1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9919-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15547276

  • £164.47 / 100 tests


EU Stock 5
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Description

Description

Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.Applications Reported: NOK-1 has been reported for use in flow cytometric analysis.Applications Tested: This NOK-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD178
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P48023
FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1, Fas Ligand, Fas-LG
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
NOK-1
PE
FASLG
Conjugated
FASLG
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
356
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