CD178 (Fas Ligand), Functional Grade, clone: NOK-1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-9919-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.Applications Reported: NOK-1 has been reported for use in flow cytometric analysis. NOK-1 has also been reported in blocking of FasL mediated killing in functional assays.Applications Tested: The NOK-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|CD178 (CD95 Ligand)|
|PBS with no preservative; pH 7.2|
|FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1, Fas Ligand, Fas-LG|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.