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CD178 (Fas Ligand), Functional Grade, clone: NOK-1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9919-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15258837

  • £206.44 / 500Āµg


EU Stock 1
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Description

Description

Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.Applications Reported: NOK-1 has been reported for use in flow cytometric analysis. NOK-1 has also been reported in blocking of FasL mediated killing in functional assays.Applications Tested: The NOK-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD178 (CD95 Ligand)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P48023
FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1, Fas Ligand, Fas-LG
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryĆ¾Functional Assay
NOK-1
Functional Grade
FASLG
Purified
FASLG
IgG1, kappa
500μg
Store at 2-8°C.
Primary
356
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