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CD177, eFluor 660, clone: MEM-166 (MEM166), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-1779-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15519916

  • £62.63 / 25 tests


Estimated Shipment date
from Supplier 23-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.015 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD177
MEM-166 (MEM166)
eFluor 660
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.015μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
neutrophil-specific antigen, NB1, NB-1
IgG1
RUO
Primary
Human
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