CD177, eFluor 660, clone: MEM-166 (MEM166), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-1779-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.015 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|HNA-2a, HNA2A, NB1, NB1 GP, PRV-1, PRV1, CD177 antigen, NB1 glycoprotein, cell surface receptor, human neutrophil alloantigen 2a, polycythemia rubra vera 1, polycythemia rubra vera protein 1|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.