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CD177, eFluor 660, clone: MEM-166 (MEM166), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-1779-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15519916

  • £62.63 / 25 tests


Estimated Shipment date
from Supplier 29-06-2018
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Description

Description

Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.015 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD177
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q8N6Q3
HNA-2a, HNA2A, NB1, NB1 GP, PRV-1, PRV1, CD177 antigen, NB1 glycoprotein, cell surface receptor, human neutrophil alloantigen 2a, polycythemia rubra vera 1, polycythemia rubra vera protein 1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
MEM-166 (MEM166)
eFluor 660
CD177
Conjugated
CD177
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
57126
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