CD172a (SIRP alpha), PE, clone: P84, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-1721-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This P84 monoclonal antibody reacts with mouse CD172a, also known as signal regulatory protein a (SIRPa). This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, macrophages, dendritic cells, but not on T and B lymphocytes. Moreover, neurons and other tissues of the central nervous system have also been shown to express CD172a. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.This monoclonal antibody has been reported to have neutralizing activity.Applications Reported: This P84 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P84 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD172a (SIRP alpha)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|SHPS-1, Signal-regulatory protein alpha-1|
For Research Use Only.