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CD172a (SIRP alpha), Functional Grade, clone: P84, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1721-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208587

  • £219.25 / 500µg


Estimated Shipment
27-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This P84 monoclonal antibody reacts with mouse CD172a, also known as signal regulatory protein a (SIRPa). This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, macrophages, dendritic cells, but not on T and B lymphocytes. Moreover, neurons and other tissues of the central nervous system have also been shown to express CD172a. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.This monoclonal antibody has been reported to have neutralizing activity.Applications Reported: This P84 antibody has been reported for use in flow cytometric analysis and neutralization assays.Applications Tested: This P84 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD172a
P84
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
SHPS-1, Signal-regulatory protein alpha-1
IgG1
RUO
Primary
Murine
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