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CD170 (Siglec F), Alexa Fluor 700, clone: 1RNM44N, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-1702-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15540357

  • £68.82 / 25µg


EU Stock 2
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Description

Description

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD170 (Siglec F)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q920G3
OB-BP2, CD33L2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
1RNM44N
Alexa Fluor 700
Siglecf
Conjugated
Siglecf
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
233186
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