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CD169 (Siglec-1), PE-eFluor 610, clone: 7-239, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 61-1699-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15570467

  • £88.05 / 25 tests


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: This 7-239 monoclonal antibody reacts with human CD169, also known as Siglec-1 and Sialoadhesin. This type I transmembrane protein is a member of the sialic acid binding Ig-like lectin (Siglec) family. Expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen in humans, CD169 binds glycoproteins and glycolipids containing N-acetylneuraminyl alpha 2-3-galactose. CD169 expression can also be induced on CD14+ monocytes by interferon-alpha and interferon-gamma to mediate HIV-1 infection. Finally, CD169 has also been shown to be involved in antigen presentation to invariant NK T cells.This monoclonal antibody has been reported to inhibit erythrocyte binding to CD169-expressing cells.Applications Reported: This 7-239 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 7-239 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD169 (Siglec-1)
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9BZZ2
CD169, SIGLEC-1, SN, dJ1009E24.1, sialic acid-binding Ig-like lectin 1, sialic acid-binding immunoglobulin-like lectin 1, sialoadhesin
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
7-239
PE-eFluor 610
SIGLEC1
Conjugated
SIGLEC1
IgG1
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
6614
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