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CD160, Functional Grade, clone: eBioCNX46-3 (CNX46-3), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1601-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288577

  • £174.50 / 500µg


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Description

Description

Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis, functional assays, immunoprecipitation, and immunoblotting (WB).Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD160
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
O88875
NK1, NK28, CD160 antigen, CD160 transmembrane isoform, CD160-delta Ig, natural killer cell receptor BY55, natural killer cell receptor
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFunctional AssayþImmunoprecipitationþWestern Blot
eBioCNX46-3 (CNX46-3)
Functional Grade
Cd160
Purified
Cd160
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
54215
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