CD16 Mouse anti-Human, Non-human primate, PerCP-eFluor 710, Clone: 3G8, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-0166-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15589086

Quantity Price
1 £ 212.4 / 100 tests
EU Stock 2
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Description and Specification

Specification

Species Reactivity Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Pigtailed Macaque, Rhesus Ma
Conjugate PerCP-eFluor 710
Concentration 5μL (0.125μg)/test
Monoclonal or Polyclonal Monoclonal
Gene Alias FcgRIII, FcgR3, ADCC receptor
Primary or Secondary Primary
Antigen CD16
Host Species Mouse
Quantity 100 tests
Applications Flow Cytometry
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Clone 3G8
Isotype IgG1
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Regulatory Status RUO

This 3G8 monoclonal antibody reacts with human and non-human primate CD16, which is also known as the low-affinity Fc gamma RIII. CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB. In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.

This monoclonal antibody has been reported to have several functional activities, including inhibition of cytotoxic ability, activation of cell signaling, and NK cell depletion in vivo. Moreover, the 3G8 antibody clone has been demonstrated to work on capuchin monkey, chimpanzee, common marmoset, cynomologous monkey, hamadyras baboon, olive baboon, pigtailed macaque, rhesus, and squirrel monkey.

Based on cross-blocking studies 3G8 recognizes the same epitope as CB16. However, 3G8 and B73.1 antibody clones bind distinct epitopes.