CD16 Mouse anti-Human, Non-human primate, Clone: 3G8, Functional Grade, eBioscience™

Mouse Monoclonal Antibody, Functional Grade

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 16-0166-85

500UG Anti-Human/Non-Human Primate CD16 Functional Grade Purified

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15527776

Quantity Price
1 £ 362.4 / 500µg
Estimated Shipment date
from Supplier 20-01-2017
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Description and Specification

Specification

Antigen CD16
Applications Flow Cytometry
Applications Functional Assay
Clone 3G8
Concentration 1mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, no sodium azide
Gene Alias FcgRIII, FcgR3, ADCC receptor
Host Species Mouse
Isotype IgG1
Quantity 500μg
Regulatory Status RUO
Species Reactivity Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Pigtailed Macaque, Rhesus Ma
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This 3G8 monoclonal antibody reacts with human and non-human primate CD16, which is also known as the low-affinity Fc gamma RIII. CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB. In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.

This monoclonal antibody has been reported to have several functional activities, including inhibition of cytotoxic ability, activation of cell signaling, and NK cell depletion in vivo. Moreover, the 3G8 antibody clone has been demonstrated to work on capuchin monkey, chimpanzee, common marmoset, cynomologous monkey, hamadyras baboon, olive baboon, pigtailed macaque, rhesus, and squirrel monkey.

Based on cross-blocking studies 3G8 recognizes the same epitope as CB16. However, 3G8 and B73.1 antibody clones bind distinct epitopes.